$@!!(JPCR$@$K$h$kDjNL$G$O!"A}I};:J*$,$"$k8BEY$r1[$($k$HA}2C$,Dd;_$7$F$7$^$$!"Cr7?NL$rH?1G$G$-$J$/$J$k$H$$$&LdBj!J%W%i%H!<8z2L!K$,$"$k!#$3$NLdBjE@$r2r7h$9$k$?$a$KMM!9$JJ}K!$,9M0F$5$l$F$*$j!"$=$N0l$D$K(Jreal-time PCR$@$,$"$k!#(Jreal-time PCR$@$H$O!"(JPCR$@$K$h$k0dEA;R$NA}I}$N2aDx$r7V8w8!CNAuCV$K$h$j%j%"%k%?%$%`$GDI@W$7!"$=$N(JPCR$@H?1~6J@~$r%W%m%U%!%$%j%s%0$9$k8!=P5;=Q$G$"$k!#H/8w$N6/EY$O(JPCR$@Cf$K9g@.$5$l$?%?!<%2%C%H(JDNA$@NL$N3d9g$r<($9!#IB5$$N?GCG$d0dEA;RAH49$(:nJ*$NDjNLJ,@O!"0l1v4pB?7?$N%9%/%j!<%K%s%0$J$I$KMxMQ$5$l$F$$$k!#(Jreal-time PCR$@$O!"EE5$1KF0$,ITMW!"A}I}$,;X?t4X?tE*$K5/$3$kNN0h$G;:J*NL$rHf3S$G$-$k!J$h$j@53N$KDjNL$G$-$k!K!"46EY$,9b$$$J$I$NMxE@$,$"$j!"?WB.$GDjNL@-$KM%$l$?J}K!$G$"$k!#$7$+$7!"5!4o$,8z2L$G%i%s%K%s%0%3%9%H$d%a%s%F%J%s%9HqMQ$J$I$,$+$+$k$H$$$&7gE@$,$"$k!#8=:_!"(Jreal-time PCR$@$K$O(JPerkin-Elmer$@
$@!Z(JTaqMan$@$K$h$k8!=P![(J
$@!!$3$NJ}K!$G$O!"L\E*$H$9$kG[Ns$rA}I}$5$;$k$?$a$N(JPCR $@%W%i%$%^!<$H!"$=$NFbB&$NG[Ns$KAjJd$9$k(JTaqMan$@%W%m!<%V$r;HMQ$9$k!#(JTaqMan$@%W%m!<%V$O!"(J5¹$@KvC<$K(JR:reporter$@!JH/8w4p!K!"(J3¹$@KvC<$K(JQ:quencher$@!J>C8w4p!K$N(J2$@$D$N7V8w?'AG$r;}$C$?(J20-24$@1v4p$N%*%j%4%L%/%l%*%A%I%W%m!<%V$G!"(JR$@$N7V8w?'AG$O(JQ$@$NF/$-$K$h$jH/8w$7$J$$$h$&$K$J$C$F$$$k!#(JPCR$@H?1~$,?J9T$7(JTaq $@%]%j%a%i!<%<$K$h$C$F%W%i%$%^!<$+$i?-D9H?1~$rB3$1$k$H!"(J5'$@"*(J3' $@%(%-%=%L%/%l%"!<%<3h@-$K$h$j(JTaqMan$@%W%m!<%V$,J,2r$5$l$k!#$3$N$H$-7V8w?'AG(JR $@$,6/$$H/8w$rH/$7!"(JTaqMan $@%7%9%F%`$G$O$3$N7V8w$r8!=P$9$k!J?^#1!K!#$^$?!"(JPCR $@H?1~$K$*$$$F(JTaqMan$@%W%m!<%V$NJ,2r$,5/$3$i$J$1$l$P!"7V8w$O8!=P$5$l$J$$$N$G!"L\E*0dEA;R$NM-L5$rB,Dj$G$-$k$3$H$K$J$k!#(J
$@
 |
||
$@?^#1!!(JTaqMan$@$N86M}(J
 |
|
$@?^#2!!A}I}$N%W%m%C%H$N%b%G%k(J